August 26, 2014 — X-ray phase-contrast imaging can provide high-quality images of objects with lower radiation dose. But until now these images have been hard to obtain and required special X-ray sources whose properties are typically only found at large particle accelerator facilities. Using a laboratory source with unprecedented brightness, scientists from the Technische Universität München (TUM), the Royal Institute of Technology in Stockholm (KTH) and University College London (UCL) have demonstrated a new approach to get reliable phase contrast with an extremely simple setup.
X-ray phase-contrast imaging is a method that uses the refraction of X-rays through a specimen instead of attenuation resulting from absorption. The images produced with this method are often of much higher quality than those based on absorption. The scientists in the team of Prof. Franz Pfeiffer are particularly interested in developing new approaches for biomedical X-ray imaging and therapy — including X-ray phase-contrast imaging. One main goal is to make this method available for clinical applications such as diagnosis of cancer or osteoporosis in the future.
In their new study, the scientists have now developed an extremely simple setup to produce X-ray phase-contrast images. The solution to many of their difficulties may seem counter-intuitive: Scramble the X-rays to give them a random structure. These speckles, as they are called in the field, encode a wealth of information on the sample as they travel through it. The scrambled X-rays are collected with a high-resolution X-ray camera, and the information is then extracted in a post-measurement analysis step.
High Accuracy and New X-ray Source
Using their new technique, the researchers have demonstrated the efficiency and versatility of their approach. "From a single measurement, we obtain an attenuation image, the phase image, but also a dark-field image," explains Dr. Irene Zanette, lead author of the publication. "The phase image can be used to measure accurately the specimen's projected thickness. The dark-field image can be just as important because it maps structures in the specimen too small to be resolved, such as cracks or fibers in materials," she adds.
The source's high brightness is also key to these results. "In the source we used a liquid metal jet as the X-ray-producing target instead of the solid targets normally used in laboratory X-ray sources," says Tunhe Zhou from KTH Stockholm, project partner of the TUM. "This makes it possible to gain the high intensity needed for phase-contrast imaging without damaging the X-ray-producing target." To obtain all images at once, an algorithm scans the speckles and analyzes the minute changes in their shape and position caused by the specimen.
But not all components of the new instrument are products of the latest cutting-edge technology. To scramble the X-rays, "we have found that a simple piece of sandpaper did the job perfectly well," adds Zanette.
The researchers are already working toward the next steps. “As a single-shot technique, speckle imaging is a perfect candidate for an efficient extension to phase-contrast tomography, which would give a three-dimensional insight into the microstructure of the investigated object,“ Zanette explains.
June 19, 2025 
