News | Radiation Therapy | February 06, 2017

Radiotargeted Therapy with SST2 Antagonists Could Combat Multiple Human Cancers

New study finds SST2 antagonists bind to greater number of SST2 sites than agonists

radiotargeted therapy, SST2 antagonists, combat cancer, neuroendocrine tumors, NETs, Journal of Nuclear Medicine study

Comparative receptor autoradiography in various cancer types (A = HE staining) with I-125-JR11 (B+C) and I-125-Tyr3-octreotide (D+E). B and D are the respective total binding, C and E the respective non-specific binding. Bars = 1 mm.

I: Breast cancer. II: Renal cell cancer. III: Medullary thyroid cancer. IV: Non-Hodgkin lymphoma. V: Colon cancer. In cancers I-V, the sections were incubated with 30’000 cpm/100 μl of antagonist or agonist. Cancers I-IV show a much higher density of sst2 with the antagonist. The colon cancer V is negative. VI: Ileal NET, incubated with 10’000 cpm/100 μl of antagonist or agonist. Credit: Jean Claude Reubi, Institute of Pathology, University of Berne, Berne, Switzerland. 

February 6, 2017 — A study published in the February issue of The Journal of Nuclear Medicine demonstrates the potential of extending peptide receptor radionuclide therapy targeting the somatostatin receptor to other types of malignancies beyond neuroendocrine tumors (NETs). The theranostic pairing of Netspot (Ga-68 DOTATATE, which is U.S. Food and Drug Administration [FDA]-approved) and Lutathera (Lu-177-DOTATATE, currently under FDA review) has already shown that it can significantly improve progression-free survival in patients with somatostatin receptor-positive NETs.

In contrast to NETs, non-NET tumors, such as breast cancers or renal cell cancers, are not routinely imaged with current somatostatin subtype 2 receptor (sst2) agonist radiotracers (which fully activate the receptor to which they bind and are subsequently internalized). This may change with sst2 antagonist tracers (which bind to the receptor but do not activate it and are not internalized). There is recent in vitro and in vivo evidence that somatostatin receptor sst2 antagonists could be even better tools to target NETs than sst2 agonists, as antagonists bind to a greater number of sst2 sites than agonists.

“The present report is the first quantitative study showing that the number of sst2 receptors that can be targeted in non-NET tumors (such as renal cell cancers or breast tumors) with a radiolabeled somatostatin antagonist can be as high as the number of somatostatin receptors being targeted in NETs with an established agonist,” explains Jean Claude Reubi, M.D., Institute of Pathology, University of Berne in Switzerland. “In other words, we can expect that non-NET tumors, which were not considered a routine indication for sst2 imaging using agonists, may now be targeted in vivo successfully with sst2 antagonists.”

In this study, the researchers compared quantitatively iodine-125 (I-125)-JR11 sst2 antagonist binding in vitro with that of the sst2 agonist I-125-Tyr3-octreotide in a variety of cancers, including prostate, breast, colon, kidney, thyroid and lymphoid tissues. NETs were included as a reference.

The researchers report that 12 of 13 breast cancers, all 12 renal cell carcinomas and all five medullary thyroid cancers demonstrated high binding of the antagonist. In contract, the agonist exhibited low binding in the majority of cases. In 15 non-Hodgkin lymphomas, many more sst2 sites were also labeled with the antagonist than with the agonist.

Other types of cancer tested were not as responsive. In 14 prostate cancers, none had sst2 binding with the agonist, and only four had a weak binding with the antagonist. None of 17 colon cancers show sst2 sites with the agonist and only three cases are weakly positive with the antagonist.

In the various tumor types, adjacent sst2-expressing tissues such as vessels, lymphocytes, nerves, mucosa or stroma were more strongly labeled with the antagonist than with the agonist. The reference NET cases, incubated with a smaller amount of tracer, were also found to have many more sst2 sites measured with the antagonist.

Reubi pointed out, “The in vitro method used in the study, somatostatin receptor autoradiography, has been shown in the past two-to-three decades to be highly predictive for the in vivo application in patients. Therefore, with the tools (sst2 antagonists) and the expertise (sst2-targeting in cancer patients) available in several clinics, one can assume that these new indications (renal cell cancers, breast tumors, etc.) can be imaged in the near future in patients.”

Rodney J. Hicks, M.D., at the Peter MaCallum Cancer Centre in Melbourne, Australia, wrote a review of the study that is also published in the February issue of The Journal of Nuclear Medicine. He noted that the results provide further impetus for the evaluation of somatostatin receptor antagonists in a range of diseases. He stated, “Since cancers can express a range of receptors, development of additional antagonists may further expand theranostic options. For clinicians, faster diagnosis, higher accuracy and stronger evidence of therapeutic effectiveness are the goal.”

For more information: www.jnm.snmjournals.org

References

Reubi, J.C., Waser, B., Maecke, H.R., Rivier, J.E. “Highly Increased 125i-Jr11 Antagonist Binding In Vitro Reveals Novel Indications for Sst2 Targeting In Human Cancers,” Journal of Nuclear Medicine. Published online Aug. 25, 2016. doi: 10.2967/jnumed.116.177733

Related Content

Heidelberg University Hospital Treats First Patient Using Carbon Ion Therapy With RayStation
News | Treatment Planning | August 23, 2019
Heidelberg University Hospital, Germany, has started using the RayStation treatment planning system for carbon ion...
Surging Demand for Image-guided, Stereotactic Radiation Therapy Driving Fiducial Marker Growth
News | Radiation Therapy | August 22, 2019
As fiducial markers gain traction in parallel to rising incidences of various cancer affecting lung, prostate, abdomen...
Videos | Treatment Planning | August 21, 2019
This is an example of the Mirada DLCExpert deep learning software that automatically identifies organs, segments and
First Cancer Patient Treated With Accuray Radixact System With Synchrony Motion Tracking
News | Image Guided Radiation Therapy (IGRT) | August 21, 2019
Physicians at the Froedtert & the Medical College of Wisconsin (MCW) Clinical Cancer Center in Milwaukee have...
Videos | Patient Positioning Radiation Therapy | August 21, 2019
This is a quick demonstration of the Varian Identify image-guided patient positioning system at the...
Videos | Treatment Planning | August 21, 2019
This is a lung cancer tumor radiotherapy...
Videos | Computed Tomography (CT) | August 21, 2019
This is a quick walk around of a mobile 32-slice...
First GammaTile Therapy Case Completed in Arizona
News | Radiation Therapy | August 19, 2019
HonorHealth Scottsdale Osborn Medical Center is the first hospital in Arizona to begin offering GammaTile Therapy, a...